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Optimisation of polyacrylamide gel composition as support for enzyme immobilisation by covalent linking
Author(s) -
Pizarro C,
GonzálezSáiz J M,
SánchezJiménez J J
Publication year - 2000
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/1097-4660(200011)75:11<1040::aid-jctb313>3.0.co;2-5
Subject(s) - covalent bond , polyacrylamide , polyacrylamide gel electrophoresis , composition (language) , chemistry , enzyme , chromatography , biochemistry , organic chemistry , polymer chemistry , linguistics , philosophy
A methodology based on experimental design to optimise a polyacrylamide gel as the support for enzyme immobilisation by covalent linking is described. A proper selection of the variables responsible for the gel structure, the amount of the linking agent, the support enzyme and suitable synthesis conditions lead to an increase in the activity retained by the gel. The maximum retained activity is chosen with the response surface methodology in terms of gel composition. © 2000 Society of Chemical Industry