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Intraparticle cell growth and cell leakage in cultures of Nicotiana tabacum cells immobilized in calcium alginate gel beads
Author(s) -
ShibasakiKitakawa Naomi,
Iizuka Yasuhiro,
Yonemoto Toshikuni
Publication year - 2000
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/1097-4660(200011)75:11<1008::aid-jctb297>3.0.co;2-t
Subject(s) - calcium alginate , leakage (economics) , chemistry , chromatography , calcium , cell , nicotiana tabacum , biochemistry , organic chemistry , gene , economics , macroeconomics
Immobilized Nicotiona tabacum cells in calcium alginate gel beads were prepared under various conditions and then were cultivated. The effects of different conditions of preparation, in relation to concentration of calcium ions (Ca 2+ ), on intraparticle cell growth and cell leakage from beads were investigated experimentally. As the amount of Ca 2+ incorporated into the beads increased, the numbers of cells leaked from the beads into the medium decreased. However, cell growth was inhibited by high Ca 2+ concentrations in the beads. Optimal conditions existed, which prevented cell leakage without inhibiting intraparticle cell growth. The effect of adding CaCl 2 to the culture medium was also studied. The Ca 2+ , used for the alginate crosslinking, gradually leached from the beads with increasing cultivation time, such that the beads gradually became brittle and fragile. The addition of CaCl 2 was effective in preventing Ca 2+ loss from the beads and cell leakage. © 2000 Society of Chemical Industry