Premium
Extraction of yeast alcohol dehydrogenase using reversed micelles formed with CTAB
Author(s) -
Zhang TianXi,
Liu HuiZhou,
Chen JiaYong
Publication year - 2000
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/1097-4660(200009)75:9<798::aid-jctb282>3.0.co;2-p
Subject(s) - bromide , pulmonary surfactant , alcohol dehydrogenase , micelle , yeast , extraction (chemistry) , cationic polymerization , chemistry , alcohol , chromatography , ionic strength , biochemistry , organic chemistry , aqueous solution
A large molecular weight protein, alcohol dehydrogenase (ADH, 141 kDa), has been purified from baker's yeast using reversed micelles formed with a cationic surfactant (cetyltrimethylammonium bromide, CTAB) using the phase transfer method. Various parameters, such as pH, ionic strength and contact time of the two phases in ADH forward and backward transfer were studied. The active ADH was successfully recovered after a full forward and backward extraction cycle. The recovery of ADH activity obtained was ∼90% and the purification factor was 3.1 for the overall process. © 2000 Society of Chemical Industry