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Lipolytic enzyme production in batch and fed‐batch cultures of Ophiostoma piceae and Fusarium oxysporum
Author(s) -
Tamerler Candan,
Keshavarz Tajalli
Publication year - 2000
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/1097-4660(200009)75:9<785::aid-jctb293>3.0.co;2-r
Subject(s) - fusarium oxysporum , lipase , food science , fermentation , chemistry , batch production , esterase , fed batch culture , fusarium , enzyme , biology , botany , biochemistry , organic chemistry
Lipase and esterase production by Ophiostoma piceae and Fusarium oxysporum were enhanced and extended by developing a fed‐batch process in stirred tank reactors. Fed‐batch strategy improved lipolytic enzyme production from Ophiostoma piceae in both 2 and 20 dm 3 stirred tank reactors. However, fed‐batch fermentation of Fusarium oxysporum in the 2 dm 3 reactor was more effective than both batch and fed‐batch fermentations in the 20 dm 3 reactor. When a medium composed of only carbon and nitrogen source was intermittently fed to the cultures, the maximum specific lipase activity was improved by more than 80% and 35% in Ophiostoma piceae and Fusarium oxysporum cultures respectively. The maximum specific esterase activity was improved by 20% and 15% in Ophiostoma piceae and Fusarium oxysporum cultures respectively. The duration of production for both fungi extended from 144 to 216 h compared with a batch culture under the same condition. © 2000 Society of Chemical Industry