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Temporal expression of growth factor genes during myogenesis of satellite cells derived from the biceps femoris and pectoralis major muscles of the chicken
Author(s) -
Kocamis Hakan,
McFarland Douglas C.,
Killefer John
Publication year - 2001
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/1097-4652(200101)186:1<146::aid-jcp1014>3.0.co;2-q
Subject(s) - myostatin , follistatin , biology , myogenesis , microbiology and biotechnology , basic fibroblast growth factor , gene expression , transforming growth factor , cell culture , messenger rna , growth factor , medicine , endocrinology , cell growth , gene , myocyte , skeletal muscle , receptor , biochemistry , genetics
The expression of mRNAs for transforming growth factors (TGF‐β2, myostatin, activin‐B, and follistatin), insulin‐like growth factors (IGF‐I and ‐II), and fibroblast growth factor (basic, bFGF) was investigated in satellite cells derived from chicken pectoralis major (PM) and biceps femoris (BF) muscles in the stages from initiation of proliferation to fusion. These growth factor gene cDNAs were synthesized by reverse transcript ase polymerase chain reaction (RT‐PCR). No myostatin, activin‐B, follistatin or bFGF expression was detected in either cell culture at 0 h. TGF‐β2 mRNA level increased at 48 h ( P < 0.01) and remained constant through 144 h in both PM and BF satellite cell cultures. The ontogeny of myostatin gene expression with the exception of a sharp increase in BF culture at 72 h ( P < 0.01), was nearly identical in both cell cultures. Activin‐B mRNA level in PM satellite cells was higher than that in BF satellite cells at 72 h and 120 h ( P < 0.01). Follistatin mRNA in PM satellite cells was higher than that in BF satellite cells at 24, 96, and 120 h culture ( P < 0.01). No IGF‐I gene expression was detected in cell cultures at any time point. IGF‐II gene expression in BF satellite cells declined at 96 h ( P < 0.01) and remained reduced until 144 h. bFGF mRNA in both satellite cell cultures increased at 24 h ( P < 0.05) and remained at this level in BF satellite cells through 144 h. J. Cell. Physiol. 186:146–152, 2001. © 2001 Wiley‐Liss, Inc.