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Growth factors and cytokines modulate gene expression of cell‐surface proteoglycans in human periodontal ligament cells
Author(s) -
Worapamorn W.,
Haase H.R.,
Li H.,
Bartold P.M.
Publication year - 2001
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/1097-4652(2001)9999:9999<000::aid-jcp1047>3.0.co;2-v
Subject(s) - syndecan 1 , microbiology and biotechnology , proteoglycan , downregulation and upregulation , cell , transforming growth factor , cell culture , gene expression , biology , cell growth , autocrine signalling , periodontal fiber , platelet derived growth factor receptor , growth factor , chemistry , receptor , extracellular matrix , gene , biochemistry , medicine , genetics , dentistry
Cell‐surface proteoglycans have been known to be involved in many functions including interactions with components of the extracellular microenvironment, and act as co‐receptors which bind and modify the action of various growth factors and cytokines. The purpose of this study was to determine the regulation by growth factors and cytokines on cell‐surface proteoglycan gene expression in cultured human periodontal ligament (PDL) cells. Subconfluent, quiescent PDL cells were treated with various concentrations of serum, bFGF, PDGF‐BB, TGF‐β1, IL‐β1, and IFN‐γ. RT‐PCR technique was used, complemented with Northern blot for syndecan‐1, to examine the effects of these agents on the mRNA expression of five cell‐surface proteoglycans (syndecan‐1, syndecan‐2, syndecan‐4, glypican and betaglycan). Syndecan‐1 mRNA levels increased in response to serum, bFGF and PDGF‐BB, but decreased in response to TGF‐β1, IL‐1β and IFN‐γ. In contrast, syndecan‐2 mRNA levels were upregulated by TGF‐β1 and IL‐1β stimulation, but remained unchanged with the other agents. Betaglycan gene expression decreased in response to serum, but was upregulated by TGF‐β1 and unchanged by the other stimulants. Additionally, syndecan‐4 and glypican were not significantly altered in response to the regulator molecules studied, with the exception that glypican is decreased in response to IFN‐γ. These data demonstrate that the gene expression of the five cell‐surface proteoglycans studied is differentially regulated in PDL cells lending support to the notion of distinct functions for these cell‐surface proteoglycans. © 2001 Wiley‐Liss, Inc.