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Pervanadate induces the hyperphosphorylation but not the activation of human heat shock factor 1
Author(s) -
Park Jeonghyeon,
Liu Alice Y.C.
Publication year - 2000
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/1097-4652(200012)185:3<348::aid-jcp5>3.0.co;2-3
Subject(s) - hyperphosphorylation , hsf1 , protein tyrosine phosphatase , kinase , chemistry , protein kinase a , phosphorylation , p38 mitogen activated protein kinases , tyrosine kinase , heat shock factor , microbiology and biotechnology , tyrosine phosphorylation , phosphatase , heat shock protein , biochemistry , signal transduction , biology , hsp70 , gene
In this study, we evaluated the effects of pervanadate, a tyrosine phosphatase inhibitor, on the regulation and function of heat‐shock factor 1 (HSF1) in HeLa cells. We showed that 50–100 μM pervanadate induced the hyperphosphorylation of the latent HSF1, as demonstrated by a retarded mobility of the HSF1 protein in SDS‐polyacrylamide gel electrophoresis and as supported by the reversal of this mobility shift upon treatment of the cell extract with acid phosphatase. Pervanadate by itself had no effect on the monomeric stoichiometry and DNA‐binding activity of HSF1. Upon heat shock, the pervanadate‐induced hyperphosphorylated HSF1 formed DNA‐binding trimers and translocated into the nuclear compartment. At high concentration (∼500 μM), pervanadate also induced the tyrosine phosphorylation of many cellular proteins and blunted the heat‐induced transcription of hsp 70. N ‐acetyl cysteine inhibited these effects of pervanadate, suggesting a redox‐based mechanism for its activity. Analysis of the activation of mitogen‐activated protein kinases (MAPKs) using antibodies specific for the phospho‐form (activated) of the kinases in Western blot showed that pervanadate activated extracellular signal‐regulated kinase (ERK1/2), c ‐Jun‐ N ‐terminal kinase 1/2 (JNK1/2), and p‐38 kinase. Pharmacological inhibitors of the ERK1/2 kinase pathway or the p38 kinase had little or no effect on the pervanadate‐induced hyperphosphorylation of HSF1. Our results show that hyperphosphorylation of hHSF1 can occur prior to and independent of other events involved in the activation of hHSF1. The possibility that activation of the MAPK signaling cascade, notably JNK, may contribute to the hyperphosphorylation of human HSF1 (hHSF1) is discussed. J. Cell. Physiol. 185:348–357, 2000. © 2000 Wiley‐Liss, Inc.