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Effect of cytokines on the proliferation/differentiation of stroma‐initiating cells
Author(s) -
Yamada Muneo,
Suzu Shinya,
TanakaDouzono Miyuki,
Wakimoto Noriko,
Hatake Kiyohiko,
Hayasawa Hirotoshi,
Motoyoshi Kazuo
Publication year - 2000
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/1097-4652(200009)184:3<351::aid-jcp9>3.0.co;2-v
Subject(s) - stromal cell , haematopoiesis , stroma , growth factor , biology , bone marrow , tumor necrosis factor alpha , macrophage colony stimulating factor , colony stimulating factor , stem cell factor , microbiology and biotechnology , granulocyte macrophage colony stimulating factor , transforming growth factor , cytokine , macrophage , stem cell , immunology , cancer research , in vitro , biochemistry , immunohistochemistry , receptor
A culture system that identifies the precursor of murine bone marrow fibroblastic stromal cells (stroma‐initiating cells, SIC) has been developed. In this system, mature fibroblasts are depleted by adherence to plastic dishes and the nonadherent cells are seeded at a low density, which results in the formation of colonies composed of fibroblastic cells. Macrophage colony‐stimulating factor (M‐CSF) has been shown to accelerate the colony formation in the system. In this study, we examined the stroma‐inducing activity of a number of cytokines. Neither granulocyte‐CSF, stem cell factor, interleukin (IL)‐1, IL‐6, transforming growth factor, epidermal growth factor, insulin‐like growth factor, platelet‐derived growth factor, nor fibroblast growth factor showed the activity. Similarly, tumor necrosis factor (TNF) did not show any stroma‐inducing activity, but the factor inhibited the stromal colony formation induced by M‐CSF. In this study, we found that granulocyte/macrophage‐CSF (GM‐CSF) and IL‐3, as well as M‐CSF had the stroma‐inducing activity. Neither an additive nor synergistic effect was observed when the three factors were assayed in various combinations. The stroma‐inducing activity of M‐CSF, GM‐CSF and IL‐3 was observed even if lineage‐negative bone marrow cells were used as target cells, suggesting that mature hematopoietic cells such as macrophages and granulocytes were not involved in the induction of stromal colony formation by these factors. Our results raise the possibility that GM‐CSF and IL‐3 as well as M‐CSF stimulate the proliferation or differentiation of the precursor of bone marrow fibroblastic stromal cells. J. Cell. Physiol. 184:351–355, 2000. © 2000 Wiley‐Liss, Inc.