Premium
Effect of the neuropeptide substance P on the rat bone marrow‐derived osteogenic cells in vitro
Author(s) -
Adamus Maria A.,
Dąbrowski Zbigniew J.
Publication year - 2001
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/1097-4644(20010601)81:3<499::aid-jcb1063>3.0.co;2-y
Subject(s) - substance p , alkaline phosphatase , endocrinology , bone marrow , medicine , chemistry , in vitro , calcium , receptor , subculture (biology) , proline , neuropeptide , biochemistry , biology , amino acid , enzyme , botany
Substance P containing, thin, sensory nerve fibres have been demonstrated in bone and bone marrow. However the role of substance P in bone tissue is not fully understood. We investigated the effects of substance P on the growth and development of rat bone marrow‐derived osteogenic cells in vitro. To examine this, the marrow‐derived osteogenic cells were treated from 3rd to 6th day of subculture with substance P at concentrations 10 −10 , 10 −9 and 10 −8 M. [ 3 H]‐thymidine, L‐2,3‐[ 3 H]‐proline incorporation, protein accumulation, alkaline phosphatase activity, and calcium deposition were measured in cultures. Substance P slightly stimulated [ 3 H]‐thymidine incorporation at 10 −10 M. Protein accumulation and L‐2,3‐[ 3 H]‐proline incorporation were enhanced in a dose dependent manner. Simultaneous application of spantide, a substance P receptor antagonist, could not block substance P‐induced L‐2,3‐[ 3 H]‐proline incorporation probably because of statistically significant effect of spantide itself. Calcium deposits were significantly lower (about 30%) in cultures treated with SP. This effect was probably due in part by the fall in alkaline phosphatase activity which in substance P treated cultures was decreased about 17%. Our results indicate that substance P could be one of the factors modulating bone metabolism. J. Cell. Biochem. 81:499–506, 2001. © 2001 Wiley‐Liss, Inc.