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Distribution of DNA replication origins between matrix‐attached and loop DNA in mammalian cells
Author(s) -
Djeliova Vera,
Russev George,
Anachkova Boyka
Publication year - 2000
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/1097-4644(20010301)80:3<353::aid-jcb80>3.0.co;2-y
Subject(s) - chinese hamster ovary cell , dna , dna replication , microbiology and biotechnology , biology , eukaryotic dna replication , scaffold/matrix attachment region , genetics , cell culture , chromatin , chromatin remodeling
Using a previously developed procedure (Gencheva et al. [1996] J Biol Chem 271:2608–2614), we isolated a DNA fraction consisting of short fragments originating from the regions of initiation of DNA synthesis from exponentially growing Chinese hamster ovary cells. This fraction arbitrarily designated as “collective origin fraction” was labeled in vitro and used to probe the abundance of origin containing sequences in preparations of matrix‐attached and loop DNA isolated by two different procedures from Chinese hamster ovary cells. Alternatively, an individual DNA replication origin sequence — a 478‐bp long DNA fragment located at about 17‐kb downstream of the dihydrofolate reductase gene — was used to probe the same matrix‐attached and loop DNA fractions. The results with both the collective and individual DNA replication origins showed that there was random distribution of the origin sequences between DNA attached to the matrix and DNA from the loops. J. Cell. Biochem. 80:353–359, 2001. © 2001 Wiley‐Liss, Inc.