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Role of the phosphorolysis of deoxyadenosine in the cytotoxic effect of the combination of deoxyadenosine and deoxycoformycin on a human colon carcinoma cell line (LoVo)
Author(s) -
Giorgelli Francesco,
Giannecchini Michela,
Bemi Valentina,
Turchi Gino,
Sgarrella Francesco,
Tozzi Maria Grazia,
Camici Marcella
Publication year - 2000
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/1097-4644(20010201)80:2<241::aid-jcb130>3.0.co;2-q
Subject(s) - deoxyadenosine , phosphorolysis , deoxycoformycin , cytotoxic t cell , chemistry , biochemistry , adenosine , purine nucleoside phosphorylase , adenosine deaminase , enzyme , in vitro , purine
In LoVo cells, phosphorolytic activity acting on deoxyadenosine plays a major role in the resistance to the cytotoxic effect of the combination of deoxynucleoside with deoxycoformycin. In fact, the observed dependence of toxicity on cell density appears to be related to the metabolic conversion of deoxyadenosine into adenine. The phosphorylation of the deoxynucleoside, which represents the first step towards the formation of the cytotoxic agent dATP, proceeds at a significantly lower rate as compared to the phosphorolysis of deoxyadenosine. The analysis of the levels of deoxyadenosine and its derivatives in the incubation media reveals that the rates of disappearance of deoxyadenosine and of formation of adenine increase in concert with the reduction of the effect on cell survival. J. Cell. Biochem. 80:241–247, 2000. © 2000 Wiley‐Liss, Inc.