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Environmental acclimatization of the carp modulates the transcription of β‐actin
Author(s) -
Sarmiento José,
Leal Sergio,
Quezada Claudia,
Kausel Gudrun,
Figueroa Jaime,
Inés Vera María,
Krauskopf Manuel
Publication year - 2000
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/1097-4644(20010201)80:2<223::aid-jcb110>3.0.co;2-o
Subject(s) - acclimatization , carp , biology , transcription (linguistics) , housekeeping gene , gene , gene expression , microbiology and biotechnology , fish <actinopterygii> , genetics , fishery , linguistics , philosophy
A cascade of mechanisms involving changes in gene expression are substantial to shape the adaptive responses that a eurythermal fish requires upon environmental changes in its habitat. We have previously shown that the cyclical reprogramming of rRNA transcription is a remarkable feature in carp under seasonal acclimatization. Using in situ hybridization and competitive RT‐PCR we found significant differences in β‐actin transcripts, generally accepted to be coded by a typical housekeeping gene, in tissues from summer‐ and winter‐acclimatized carp. The physiological differential β‐actin transcription herein reported places us on the alert for the reference genes estimated to be constitutive to quantitatively assess gene transcripts. J. Cell. Biochem. 80:223–228, 2000. © 2000 Wiley‐Liss, Inc.