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Hypoxia induces differential expression of the integrin receptors α vβ3 and α vβ5 in cultured human endothelial cells
Author(s) -
Walton Harry L.,
Corjay Martha H.,
Mohamed Seema N.,
Mousa Shaker A.,
Santomenna Linda D.,
Reilly Thomas M.
Publication year - 2000
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/1097-4644(20000915)78:4<674::aid-jcb16>3.0.co;2-g
Subject(s) - angiogenesis , umbilical vein , northern blot , messenger rna , blot , integrin , hypoxia (environmental) , microbiology and biotechnology , biology , western blot , receptor , endothelial stem cell , chemistry , biochemistry , gene , in vitro , cancer research , organic chemistry , oxygen
The integrins α vβ3 and α vβ5 have been implicated in playing a key role in the process of angiogenesis. In this study, we examined the effects of hypoxia, an important stimulus of angiogenesis, on the differential expression of the integrin subunits β 3 and β 5 . β 3 and β 5 messenger RNA (mRNA), protein levels, and α v β 3 function were measured in human umbilical vein endothelial cells (HUVECs) cultured under normoxic and hypoxic (1% O 2 ) conditions. Cells exposed to hypoxic conditions for up to 72 h showed gradually increased mRNA levels of α V and β 3 , peaking at 24 h, in comparison with cells cultured under normoxic conditions. However, β 5 mRNA levels, under the same hypoxic conditions, remained at a constant level. Results from Western blot analysis of HUVECs, cultured under hypoxic conditions, paralleled those of the Northern analysis with an increased expression in α v β 3 protein levels, measured by blotting with LM609, evident by 24 h. α v β 5 protein levels, measured by blotting with P1F6, did not change for up to 72 h. HUVECs cultured under hypoxic conditions for 72 h showed increased attachment to fibrinogen, an α v β 3 mediated process. These results indicate that hypoxia can increase expression of α v β 3 in HUVECs, and that hypoxic regulation of α v β 3 may be an important regulator of angiogenesis. J. Cell. Biochem. 78:674–680, 2000. © 2000 Wiley‐Liss, Inc.