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Hydrogen peroxide decelerates recovery of action potential after high‐frequency fatigue in skeletal muscle
Author(s) -
Oba Toshiharu,
Ishikawa Tatsuya,
Takaishi Tetsuo,
Aoki Takako,
Yamaguchi Mamoru
Publication year - 2000
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/1097-4598(200010)23:10<1515::aid-mus7>3.0.co;2-i
Subject(s) - stimulation , skeletal muscle , catalase , hydrogen peroxide , chemistry , membrane potential , sartorius muscle , muscle fatigue , compound muscle action potential , medicine , biophysics , endocrinology , electrophysiology , biochemistry , electromyography , oxidative stress , biology , physical medicine and rehabilitation
Effects of reactive oxygen species (ROS), especially hydrogen peroxide (H 2 O 2 ), on recovery of action potential by resting for 30 min after high‐frequency fatigue were studied using frog skeletal muscle fibers. After stimulation at a frequency of 50 H Z for 2 min, the action potential amplitude was decreased by 14.5 mV from controls, and resting membrane was depolarized by 15.4 mV. Action potential duration was also prolonged by high‐frequency stimulation (1.5 ms in controls to 2.6 ms). The high‐frequency stimulation used here caused no muscle damage. The action potential was partially improved after a 30‐min rest. Addition of catalase at 500 units/ml or H 2 O 2 at 0.5 mM to sartorius muscle did not alter any of the parameters of the action potential after high‐frequency stimulation. Treatment with catalase accelerated post‐fatigue recovery of the action potential. Application of H 2 O 2 delayed post‐fatigue recovery of resting and action potentials. When added to detubulated toe muscle fibers, catalase no longer improved the attenuation of action potential induced by high‐frequency stimulation, even after a 30‐min rest. These findings suggest that removal of H 2 O 2 from transverse tubules is effective for post‐fatigue recovery of action potential in skeletal muscle. © 2000 John Wiley & Sons, Inc. Muscle Nerve 23: 1515–1524, 2000.