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Effects of LIF dose and laminin plus fibronectin on axotomized sciatic nerves
Author(s) -
Dowsing Bruce J.,
Hayes Alan,
Bennett Timothy M.,
Morrison Wayne A.,
Messina Aurora
Publication year - 2000
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/1097-4598(200009)23:9<1356::aid-mus6>3.0.co;2-2
Subject(s) - leukemia inhibitory factor , laminin , sciatic nerve , fibronectin , gastrocnemius muscle , extracellular matrix , regeneration (biology) , axon , chemistry , sciatic nerve injury , medicine , endocrinology , anatomy , cytokine , biology , interleukin 6 , skeletal muscle , microbiology and biotechnology , biochemistry
Leukemia inhibitory factor (LIF), a cytokine which has neurotrophic and myotrophic activities, has been shown to enhance nerve regeneration and consequent return of muscle function in the entubulation model of sciatic nerve repair. Fibronectin (FN) and laminin (LN) are two extracellular matrix (ECM) components that, when combined, promote axon growth in the entubulation model. The aim of this study was to determine the optimal LIF dose and the efficacy of FN plus LN administered either alone or simultaneously with the optimal LIF dose. We found that at 12 weeks following nerve repair, a single 10 ng LIF dose produced the largest medial gastrocnemius (MG) muscle mass ( P < 0.0001) and maximum force contraction ( P < 0.001). The diameter of the axons in the FN plus LN group were significantly greater than for saline ( P < 0.001) and the LIF dose groups ( P < 0.01). When 10 ng LIF was combined with FN plus LN, the MG muscle mass was significantly greater than the optimal LIF dose ( P < 0.05), suggesting an additive effect. Our findings support the view that combinations of factors, which perhaps act on complementary mechanisms for nerve regeneration, will be required to maximally potentiate nerve regeneration and return of muscle function after nerve injury. © 2000 John Wiley & Sons, Inc. Muscle Nerve 23: 1356–1364, 2000.