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Cell death in vasculitic neuropathy
Author(s) -
Heuss Dieter,
ProbstCousin Stefan,
Kayser Corinna,
Neundörfer Bernhard
Publication year - 2000
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/1097-4598(200007)23:7<999::aid-mus1>3.0.co;2-i
Subject(s) - fas ligand , peripheral blood mononuclear cell , tunel assay , granzyme , apoptosis , granzyme b , pathology , medicine , peripheral neuropathy , cd8 , immunohistochemistry , programmed cell death , immunology , biology , antigen , endocrinology , perforin , biochemistry , in vitro , diabetes mellitus
Vasculitic neuropathy shows cluster of differentiation (CD)4+ and CD8+ perivascular infiltrates. CD8+ cells kill the target by different ways. Some mechanisms require the release of granzymes and T‐cell restricted intracellular antigen (TIA‐1) which induce cell death by apoptosis. A nonsecretory ligand‐mediated mechanism has been proposed for apoptosis and requires the interaction of Fas and Fas‐ligand. We studied apoptosis by immunohistochemistry and terminal deoxynucleotidyl transferase‐mediated nick end labeling (TUNEL) in nerve biopsy specimens in 19 cases of vasculitic neuropathy and 8 controls. In vasculitic neuropathy, perivascular mononuclear cells expressed TIA‐1, granzyme A, and granzyme B. Regarding the ligand‐mediated mechanism, we observed Fas+ and Fas‐ligand+ mononuclear cells in an epineurial‐perivascular distribution. Cells undergoing apoptosis, as demonstrated by TUNEL, represented mononuclear cells. In vasculitic neuropathy, apoptosis by both a secretory and a nonsecretory mechanism is suspected. However, apoptosis seems to be restricted to inflammatory mononuclear cells, suggesting that it may play a critical role in recovery from vasculitic neuropathy. © 2000 John Wiley & Sons, Inc. Muscle Nerve 23:999–1004, 2000