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Identification of trifluoperazine metabolites using HPLC–NMR of test mixtures
Author(s) -
Dachtler Markus,
Händel Heidi,
Glaser Tobias,
Lindquist Diana,
Hawk Roger M.,
Karson Craig N.,
Komoroski Richard A.,
Albert Klaus
Publication year - 2000
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/1097-458x(200011)38:11<951::aid-mrc762>3.0.co;2-6
Subject(s) - trifluoperazine , chemistry , chromatography , high performance liquid chromatography , nuclear magnetic resonance spectroscopy , proton nmr , silica gel , metabolite , stereochemistry , calmodulin , biochemistry , enzyme
The metabolites of the antipsychotic drug trifluoperazine (TFP) were investigated with on‐line coupling of high‐performance liquid chromatography (HPLC) and NMR spectroscopy. A chromatographic method was developed using a reversed‐phase C 30 silica gel column. Rats were given a cumulative dose of 90 mg kg −1 TFP dihydrochloride and samples of brain and muscle tissues were extracted after 29 h. The peaks obtained on chromatographic separation were assigned to trifluoperazine and its metabolites. With the help of chromatograms obtained from test mixtures, 1D and 2D HPLC–NMR were used to identify the peaks found for these mixtures. Copyright © 2000 John Wiley & Sons, Ltd.