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New methods for measuring 1 H– 31 P coupling constants in nucleic acids
Author(s) -
Gotfredsen C. H.,
Meissner A.,
Duus J. Ø.,
Sørensen O. W.
Publication year - 2000
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/1097-458x(200008)38:8<692::aid-mrc723>3.0.co;2-o
Subject(s) - chemistry , heteronuclear molecule , dephasing , coupling constant , histone octamer , nucleic acid , pulse sequence , dna , pulse (music) , nuclear magnetic resonance , stereochemistry , nuclear magnetic resonance spectroscopy , quantum mechanics , physics , histone , biochemistry , voltage , nucleosome
New pulse sequences for the measurement of 1 H– 31 P coupling constants in the backbone of DNA duplexes are presented. They have a resemblance to HMBC but the constant J dephasing delay is exploited for heteronuclear J modulation so that the peaks in the two‐dimensional spectrum appear as doublets with splittings scaled relative to the 31 P chemical shifts. The new pulse sequences have been applied to a self‐complementary octamer DNA duplex 5′‐CGCTAGCG‐3′ (CTAG). Copyright © 2000 John Wiley & Sons, Ltd.