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Fluorine as an NMR probe for structural studies of chemical and biological systems †
Author(s) -
Gakh Yelena G.,
Gakh Andrei A.,
Gronenborn Angela M.
Publication year - 2000
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/1097-458x(200007)38:7<551::aid-mrc686>3.0.co;2-q
Subject(s) - chemistry , fluorine , fluorine 19 nmr , molecule , macromolecule , coupling constant , chemical physics , computational chemistry , chemical shift , biomolecule , j coupling , nuclear magnetic resonance spectroscopy , organic chemistry , biochemistry , physics , particle physics
Applications of fluorine NMR to probe long‐range interactions in rigid organic molecules, namely cubanes, and to study structures and dynamics of proteins and other biological systems, are presented. 19 F NMR parameters, especially long‐range coupling constants are shown to reveal intrinsic features of the molecules. Correlations between long‐range (more than three‐bond) couplings involving fluorine and other chemical and physico‐chemical properties of the compounds are demonstrated, and examples of unusual couplings which cannot be explained by existing theories of ‘through‐bond’ and ‘through‐space’ mechanisms are discussed. Recent applications of fluorine labeling of aromatic and aliphatic amino acids in investigating protein structures and dynamics are highlighted. The potential of using 19 F NMR parameters, such as large spin–spin coupling constants observed for organic compounds, for structural analysis of biological macromolecules is outlined. Copyright © 2000 John Wiley & Sons, Ltd.