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Hydrogen radioisotopic labelling studies using muonium: properties of thiyl radicals potentially relevant to cellular membrane damage
Author(s) -
Rhodes Christopher J.,
Dintinger Timothy C.,
Hinds Chantal S.,
Morris Harry,
Reid Ivan D.
Publication year - 2000
Publication title -
magnetic resonance in chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.483
H-Index - 72
eISSN - 1097-458X
pISSN - 0749-1581
DOI - 10.1002/1097-458x(200006)38:13<::aid-mrc698>3.0.co;2-n
Subject(s) - chemistry , muonium , radical , radiolysis , photochemistry , aqueous solution , reaction rate constant , kinetics , muon spin spectroscopy , hydrogen , muon , organic chemistry , physics , particle physics , quantum mechanics
Thiyl radicals (RS · ) are formed labelled with muonium atoms (a radioisotope of hydrogen with a positive muon as the nucleus) and detected using the muon spin rotation (MuSR) method. This approach is shown to be extremely effective in the study of thiyl radicals, in non‐aqueous (membrane‐like) environments, from which details of the structures of these reactive free radicals and their reaction kinetics were determined. In this regard, the method is superior to conventional ESR spectroscopy, to which thiyl radicals are undetectable in liquid solution, and to pulse‐radiolysis experiments, which are limited to aqueous media; the rate constants for the reactions of thiyl radicals with lipids but also with antioxidants such as β‐carotene and (SH)glutathione are found to be enhanced in non‐aqueous media. Copyright © 2000 John Wiley & Sons, Ltd.