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Study of the structural stability of the mini‐hairpin d(GCGAAGC) by hydrogen–deuterium exchange kinetics
Author(s) -
Chraïbi Zakaria,
Réfrégiers Matthieu,
Jollès Béatrice,
Laigle Alain
Publication year - 2000
Publication title -
journal of raman spectroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.748
H-Index - 110
eISSN - 1097-4555
pISSN - 0377-0486
DOI - 10.1002/1097-4555(200006)31:6<481::aid-jrs560>3.0.co;2-0
Subject(s) - nuclease , chemistry , raman spectroscopy , kinetics , thermal stability , base pair , hydrogen–deuterium exchange , deuterium , resonance raman spectroscopy , dna , crystallography , hydrogen , stereochemistry , biochemistry , physics , organic chemistry , quantum mechanics , optics
In order to protect them against enzymatic attack of serum in the antisense strategy, oligodeoxyribonucleotides can be protected on their 3′‐side by the sequence d(GCGAAGC), which spontaneously forms a hairpin known for its extraordinary stability with regard to thermal denaturation or nuclease degradation. It is stabilized through two GC base pairs and a non‐Watson–Crick Genbond A pair. Its proton exchange half‐time as determined by resonance Raman spectroscopy is 6 days, which suggests a drastically more stable structure than that of the already very rigid canonical Z‐DNA form. Copyright © 2000 John Wiley & Sons, Ltd.