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Neurofilament mRNAs are present and translated in the normal and severed sciatic nerve
Author(s) -
SoteloSilveira José R.,
Calliari Aldo,
Kun Alejandra,
Benech Juan Claudio,
Sanguinetti Carlos,
Chalar Cora,
Sotelo José R.
Publication year - 2000
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/1097-4547(20001001)62:1<65::aid-jnr7>3.0.co;2-z
Subject(s) - neurofilament , sciatic nerve , axon , in situ hybridization , axoplasm , nerve injury , axoplasmic transport , anatomy , messenger rna , myelin , western blot , northern blot , biology , microbiology and biotechnology , chemistry , pathology , central nervous system , neuroscience , medicine , immunohistochemistry , biochemistry , gene
Local protein synthesis within axons has been studied on a limited scale. In the present study, several techniques were used to investigate this synthesis in sciatic nerve, and to show that it increases after damage to the axon. Neurofilament (NF) mRNAs were probed by RT‐PCR, Northern blot and in situ hybridization in axons of intact rat sciatic nerve, and in proximal or distal stumps after sciatic nerve transection. RT‐PCR demonstrated the presence of NF‐L, NF‐M and NF‐H mRNAs in intact sciatic nerve, as well as in proximal and distal stumps of severed nerves. Northern blot analysis of severed nerve detected NF‐L and NF‐M, but not NF‐H. This technique did not detect the three NFs mRNAs in intact nerve. Detection of NF‐L and NF‐M mRNA in injured nerve, however, indicated that there was an up‐regulation in response to nerve injury. In situ hybridization showed that NF‐L mRNA was localized in the Schwann cell perinuclear area, in the myelin sheath, and at the boundary between myelin sheath and cortical axoplasm. RNA and protein synthesizing activities were always greater in proximal as compared to distal stumps. NF triplet proteins were also shown to be synthesized de novo in the proximal stump. The detection of neurofilament mRNAs in nerves, their possible upregulation during injury and the synthesis of neurofilament protein triplet in the proximal stumps, suggest that these mRNAs may be involved in nerve regeneration, providing a novel point of view of this phenomenon. J. Neurosci. Res. 62:65–74, 2000. © 2000 Wiley‐Liss, Inc.