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Cholinergic expression by a neural stem cell line grafted to the adult medial septum/diagonal band complex
Author(s) -
Doering Laurie C.,
Snyder Evan Y.
Publication year - 2000
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/1097-4547(20000915)61:6<597::aid-jnr3>3.0.co;2-l
Subject(s) - choline acetyltransferase , cholinergic , biology , stem cell , microbiology and biotechnology , cholinergic neuron , neural stem cell , diagonal band of broca , neuroscience , neuroepithelial cell , glial fibrillary acidic protein , cellular differentiation , immunology , immunohistochemistry , biochemistry , gene
The potential of a neural stem cell line to acquire cholinergic characteristics was studied in transplants injected into the septum/diagonal band nuclei of young adult rats and mice. The stem cells integrated within the nuclei and survived for up to 9 months. Three methods were used to identify the grafted cells and to show differentiation into astrocytes and neurons. Enhanced survival of the stem cells occurred in the host brain with a previous lesion of the fimbria‐fornix pathway. Differentiated cells acquired neuronal‐like features including the expression of neurofilament subunits. In lesioned hosts, subpopulations of the grafted cells acquired a cholinergic neuronal phenotype and expressed choline acetyltransferase and the p75 neurotrophin receptor. Cells that developed into astrocytes were often associated with blood vessels and expressed glial fibrillary acidic protein. The results further exemplify the potential of stem cell lines and the property of site‐specific differentiation when this line is transplanted to the cholinergic system of the adult brain. J. Neurosci. Res. 61:597–604, 2000. © 2000 Wiley‐Liss, Inc.