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Differential expression of delta opioid receptors and mRNA in proliferating astrocytes during the cell cycle
Author(s) -
Persson P. Anders I.,
Thorlin Thorleif,
Rönnbäck Lars,
Hansson Elisabeth,
Eriksson Peter S.
Publication year - 2000
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/1097-4547(20000815)61:4<371::aid-jnr3>3.0.co;2-h
Subject(s) - mitosis , δ opioid receptor , messenger rna , microbiology and biotechnology , biology , opioid receptor , receptor , cell cycle , cerebral cortex , gene expression , cell , endocrinology , opioid , gene , genetics
Previous immunohistochemical and radioligand binding studies have shown a cell cycle‐dependent regulation of the delta opioid receptor (DOR). The relationship between DOR expression and mitosis in primary astroglial cultures of rat cerebral cortex was investigated in this study. The cultures were arrested during the G 1 /S transition or during mitosis. The DOR protein level increased twofold ( P = 0.009) during mitosis and DOR mRNA level increased threefold ( P = 0.002) during the G 1 /S transition compared to nonsynchronized cultures. DOR mRNA was also elevated (1.6‐fold, P = 0.008) during the G 1 /S transition compared with mitotic cells. A premitotic increase in DOR mRNA suggests that elevated DOR protein levels during mitosis might be regulated during transcription. J. Neurosci. Res. 61:371–375, 2000. © 2000 Wiley‐Liss, Inc.