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Fibroblast growth factor‐9 modulates the expression of myelin related proteins and multiple fibroblast growth factor receptors in developing oligodendrocytes
Author(s) -
Cohen Rick I.,
Chandross Karen J.
Publication year - 2000
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/1097-4547(20000801)61:3<273::aid-jnr5>3.0.co;2-i
Subject(s) - fibroblast growth factor receptor , fibroblast growth factor , myelin , myelin basic protein , microbiology and biotechnology , biology , fibroblast growth factor receptor 1 , fibroblast growth factor receptor 4 , receptor , endocrinology , biochemistry , central nervous system
The effect of fibroblast growth factor (FGF)‐9 on the expression of FGF receptors (FGFR) and the major myelin proteins was examined in cultures of developing rat brain oligodendrocytes (OLs), using immunological techniques. FGFR‐1, ‐3, and ‐4 were expressed at all developmental stages but were not present in isolated myelin fractions. By contrast, FGFR‐2 protein was predominantly localized to differentiating cells and myelin. FGF‐9 altered FGFR and myelin protein levels during OL differentiation; there was increased expression of FGFR‐1 and decreased levels of both FGFR‐2 and myelin proteins. Further, FGF‐9 stimulated mitogen‐associated protein kinase (MAPK) phosphorylation. The effect of FGF‐9 on MAPK, however, was transient and less robust in progenitor cells than in differentiated oligodendrocytes. The effects of FGF‐9 and FGF‐2 on FGFR and myelin protein levels were comparable; both up‐regulated FGFR‐1, and down‐regulated FGFR‐2, CNP, PLP and MBP. These findings suggest that FGF‐9 may be important for glial cell development. J. Neurosci. Res. 61:273–287, 2000. Published 2000 Wiley‐Liss, Inc.