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Overexpression of p53 in squamous cell carcinoma of the tongue in young patients with no known risk factors is not associated with mutations in exons 5–9
Author(s) -
Lingen Mark W.,
Chang KouWei,
McMurray Scott J.,
Solt Dennis B.,
Kies Merrill S.,
Mittal Bharat B.,
Haines G. Kenneth,
Pelzer Harold J.
Publication year - 2000
Publication title -
head and neck
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 127
eISSN - 1097-0347
pISSN - 1043-3074
DOI - 10.1002/1097-0347(200007)22:4<328::aid-hed3>3.0.co;2-r
Subject(s) - exon , immunohistochemistry , malignancy , gene , basal cell , biology , tumor suppressor gene , tongue , cancer research , carcinoma , cell , population , mutation , oncology , pathology , medicine , genetics , carcinogenesis , environmental health
Background This study investigated the status of the p53 tumor suppressor gene in patients less than 40 years of age who had squamous cell carcinoma of the tongue develop with no known risk factors. Methods Histologic sections from 21 patients were prepared from formalin‐fixed, paraffin‐embedded tissue and were processed for standard immunohistochemistry for detection of the p53 protein. In addition, tumors were evaluated by single‐strand conformation polymorphism and by DNA sequencing to identify potential mutations in the conserved exons (5–9) of the p53 gene. Results Eighty‐one percent (17 of 21) of the patients overexpressed p53 by immunohistochemical analysis. However, none of these patients demonstrated mutations in exons 5–9 of the gene. Conclusions These data suggest that the molecular mechanisms by which the young individuals with no risk factors had altered p53 function in oral squamous cell carcinoma may differ from those of the more typical population of individuals who have this malignancy develop. © 2000 John Wiley & Sons, Inc. Head Neck 22: 328–335, 2000.