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Analysis of three‐dimensional architecture in Feulgen‐stained fine‐needle liver aspirates, using confocal scanning laser microscopy
Author(s) -
Boon Mathilde E.,
Barlow Yvonne,
Marres Elisabeth M.,
Zeppa Pio,
Kok Lambrecht P.
Publication year - 2000
Publication title -
diagnostic cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.417
H-Index - 65
eISSN - 1097-0339
pISSN - 8755-1039
DOI - 10.1002/1097-0339(200008)23:2<82::aid-dc3>3.0.co;2-5
Subject(s) - confocal , pathology , medicine , confocal microscopy , feulgen stain , hepatocellular carcinoma , biopsy , confocal laser scanning microscopy , staining , biomedical engineering , biology , optics , physics , cancer research , microbiology and biotechnology
Early and accurate diagnosis of hepatocellular carcinoma in liver nodules using fine‐needle aspiration biopsy can still pose difficulties, particularly in cirrhotic nodules containing dysplastic hepatocytes. Loss of architectural clues, which may aid diagnosis, can be overcome by using confocal scanning laser microscopic examination of tissue fragments in smears without the need for further processing of slides. The basic fuchsin of the Feulgen method resulted in excellent confocal images without the need for further processing of the smears. Clear morphological differences in three‐dimensional reconstructions of optically sectioned tissue fragments were demonstrated in normal, hyperplastic, and malignant smears as an aid to diagnosis. Diagn. Cytopathol. 23:82–86, 2000. © 2000 Wiley‐Liss, Inc.