Open AccessUsing light scatter signal to estimate bacterial biovolume by flow cytometry
Author(s) -
Bouvier Thierry,
Troussellier Marc,
Anzil Adriana,
Courties Claude,
Servais Pierre
Publication year - 2001
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/1097-0320(20010701)44:3<188::aid-cyto1111>3.0.co;2-c
Subject(s) - bacterial cell structure , flow cytometry , light scattering , cell size , biology , cytometry , bacteria , scattering , optics , physics , microbiology and biotechnology , genetics
Background In the past decade, flow cytometry has become a useful and precise alternative to microscopic bacterial cell counts in aquatic samples. However, little evidence of its usefulness for the evaluation of bacterial biovolumes has emerged in from the literature. Methods The light scattering and cell volume of starved bacterial strains and natural bacterial communities from the Black Sea were measured by flow cytometry and epifluorescence microscopy, respectively, in order to establish a relationship between light scattering and cell volume. Results With the arc‐lamp flow cytometer, forward angle light scatter (FALS) was related to cell size in both the starved strains and natural communities, although regression parameters differed. We tested the predictive capacity of the FALS verous cell size relationship in a bacterial community from the North Sea. That analysis showed that a reliable bacterial biovolume prediction of a natural bacterial community can be obtained from FALS using a model generated from natural bacterial community data. Conclusions Bacterial biovolume is likely to be related to FALS measurements. It is possible to establish a generally applicable model derived from natural bacterial assemblages for flow cytometric estimation of bacterial biovolumes by light scatter. Cytometry 44:188–194, 2001. © 2001 Wiley‐Liss, Inc.