Open AccessMuscarinic receptor‐induced calcium responses in astroglia
Author(s) -
Catlin Michelle C.,
Kavanagh Terrance J.,
Costa Lucio G.
Publication year - 2000
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/1097-0320(20001001)41:2<123::aid-cyto7>3.0.co;2-1
Subject(s) - muscarinic acetylcholine receptor , calcium , neuroscience , chemistry , muscarinic acetylcholine receptor m3 , receptor , pharmacology , biology , biochemistry , organic chemistry
Background The objective of this study was to characterize and quantitate the calcium responses to cholinergic stimulation in individual primary rat cortical astrocytes and human 132 1N1 astrocytoma cells. Materials and Methods The fluorescent calcium probe Indo‐1 AM and an attached cell analysis and sorting (ACAS) instrument were used to quantitate calcium responses in these cells. Results A concentration‐dependent response to carbachol was seen in both cell types. However, carbachol was more potent and efficacious, and the response was more homogeneous in the cell line. The calcium response was mediated by the M3 subtype of muscarinic receptors. Experiments in the absence of extracellular calcium and with EGTA demonstrated that the initial calcium spike was due to calcium release from intracellular calcium stores, whereas the sustained elevation and oscillations were dependent on calcium influx. Protein kinase C exerts a feedback inhibition of these calcium responses, and appears to be involved in maintaining the elevated calcium concentration and oscillations. Conclusions This study provided a detailed quantitation of the changes in intracellular calcium evoked in individual astroglial cells by activation of M3 muscarinic receptors. This will allow for the study of pharmacological and toxicological agents on this response. Cytometry 41:123–132, 2000 © 2000 Wiley‐Liss, Inc.