Open AccessA rapid measurement of apoptosis‐associated light scatter changes using a hematology analyzer
Author(s) -
Lertworasirikul Thawatchai,
Bunyaratvej Ahd
Publication year - 2000
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/1097-0320(20000615)42:3<215::aid-cyto8>3.0.co;2-3
Subject(s) - apoptosis , camptothecin , flow cytometry , annexin , cytometry , fluorescein isothiocyanate , population , microbiology and biotechnology , rhodamine 123 , biology , chemistry , medicine , biochemistry , fluorescence , physics , optics , environmental health , multiple drug resistance , antibiotics
The alternative application of an automated hematology analyzer, H∗3 system, has been described for the detection of apoptosis. Apoptosis induction by the topoisomerase I inhibitor, camptothecin (CAM) on several cell lines is followed by typical morphological alterations. On the H∗3 cytogram, measurement of CAM‐treated cells revealed an increased population of cells with reduced size suggesting cell contraction during apoptosis. The decreased LUC/Lymph ratio also indicated the enhanced degree of apoptosis directly correlated with increasing CAM concentration and/or incubation period. Quantitative analysis shows a good correlation between the H∗3 measurement and flow cytometry measurements of Annexin V‐fluorescein isothiocyanate‐labeled method. Thus, the H∗3 measurement, under an appropriate adjustment, can be used as a rapid monitor for evaluating the degree of apoptotic changes in drug susceptibility testing of homogeneous cell samples. Cytometry (Comm. Clin. Cytometry) 42:215–217, 2000. © 2000 Wiley‐Liss, Inc.