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Optimization of production and downstream processing of the almond β‐glucosidase‐mediated glucosylation of glycerol
Author(s) -
De Roode Mattheus,
Peters Sjors W. P. G.,
Franssen Maurice C. R.,
Van Padt Albert Der,
De Groot Aede,
Boom Remko M.
Publication year - 2001
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/1097-0290(20010305)72:5<568::aid-bit1021>3.0.co;2-j
Subject(s) - glycerol , glucoside , yield (engineering) , chemistry , substrate (aquarium) , chromatography , enzyme , molar ratio , catalysis , biochemistry , thermodynamics , biology , medicine , ecology , physics , alternative medicine , pathology
This article describes the synthesis of glyceryl glucoside from glycerol and glucose with almond β‐glucosidase as the catalyst. A yield of 54% (0.45 mmol/g) was obtained. The influence of the enzyme stability, the water concentration, and the water activity on the glucoside yield were determined. A molar fraction‐based equilibrium constant of 2.4 ± 0.6 was found, with which the glucoside yield could be calculated for all possible combinations of initial substrate and water fractions in the reaction mixture. A model was used to optimize the glucoside yield while minimizing one of the substrate concentrations at equilibrium. This straightforward model gives a good prediction of the measured glucoside yield, according to a parity plot. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 72: 568–572, 2001.