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Horseradish peroxidase catalyzed degradation of industrially important dyes
Author(s) -
Bhunia Anirban,
Durani Susheel,
Wangikar Pramod P.
Publication year - 2001
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/1097-0290(20010305)72:5<562::aid-bit1020>3.0.co;2-s
Subject(s) - horseradish peroxidase , chemistry , peroxidase , substrate (aquarium) , phenol , phenols , degradation (telecommunications) , catalysis , phenol red , enzyme , organic chemistry , kinetics , nuclear chemistry , telecommunications , oceanography , computer science , geology , physics , quantum mechanics
Horseradish peroxidase (HRP) is known to degrade certain recalcitrant organic compounds such as phenol and substituted phenols. Here, for the first time we have shown HRP to be effective in degrading and precipitating industrially important azo dyes. For Remazol blue, the enzyme activity was found to be far better at pH 2.5 than at neutral pH. In addition, Remazol blue acts as a strong competitive inhibitor of HRP at neutral pH. Horseradish peroxidase shows broad substrate specificity toward a variety of azo dyes. Kinetic constants (K m app and V max app ) for two different dyes have been determined. In addition to providing a systematic analysis of the potential of HRP in degradation of dyes, this study opens up a new area on exploration of commercial dyes as inhibitors of enzymes. 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 72: 562–567, 2001.