Premium
On‐line determination of animal cell concentration
Author(s) -
Ducommun P.,
Bolzonella I.,
Rhiel M.,
Pugeaud P.,
von Stockar U.,
Marison I. W.
Publication year - 2001
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/1097-0290(20010305)72:5<515::aid-bit1015>3.0.co;2-q
Subject(s) - substrate (aquarium) , limiting , cell culture , chromatography , chemistry , biophysics , analytical chemistry (journal) , biology , ecology , mechanical engineering , engineering , genetics
A new approach for the indirect determination of cell concentration in the case of nonconstant metabolic rates has been developed. The specific glucose‐uptake rate was shown to be nonconstant in batch cultures of free suspended and immobilized CHO SSF3 cells. Time‐independent models correlating the specific rate to the limiting substrate concentration were established, thus providing a continuous determination of the specific rate through on‐line measurement of the limiting substrate. The method could be applied to determine on‐line cell concentration in both free suspended and immobilized cell cultures. Results were verified off‐line by crystal violet nuclei counting. The predicted cell concentration was in very good agreement with the off‐line reference during the whole exponential‐growth phase, until the specific glucose‐uptake rate tended to zero. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 72: 515–522, 2001.