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Low‐cost microbioreactor for high‐throughput bioprocessing
Author(s) -
Kostov Yordan,
Harms Peter,
RandersEichhorn Lisa,
Rao Govind
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/1097-0290(20010205)72:3<346::aid-bit12>3.0.co;2-x
Subject(s) - bioprocess , bioreactor , fermentation , chemistry , volume (thermodynamics) , throughput , optical density , chromatography , chemical engineering , food science , computer science , organic chemistry , medicine , telecommunications , physics , quantum mechanics , engineering , wireless , ophthalmology
The design of a microbioreactor is described. An optical sensing system was used for continuous measurements of pH, dissolved oxygen, and optical density in a 2 mL working volume. The K L a of the microbioreactor was evaluated under different conditions. An Escherichia coli fermentation in both the microbioreactor and a standard 1 L bioreactor showed similar pH, dissolved oxygen, and optical density profiles.%The low cost of the microbioreactor, detection system, and the small volume of the fermentation broth provide a basis for development of a multiple‐bioreactor system for high‐throughput bioprocess optimization. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 72: 346–352, 2001.