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Partial degradation of p ‐aminoazobenzene by a defined mixed culture of Bacillus subtilis and Stenotrophomonas maltophilia
Author(s) -
Zissi Urania,
Lyberatos Gerasimos
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/1097-0290(20010105)72:1<49::aid-bit7>3.0.co;2-x
Subject(s) - biodegradation , stenotrophomonas maltophilia , chemostat , bacillus subtilis , stenotrophomonas , chemistry , aniline , mineralization (soil science) , bioreactor , cometabolism , bacteria , microbiology and biotechnology , bioremediation , organic chemistry , biology , biochemistry , nitrogen , 16s ribosomal rna , gene , pseudomonas aeruginosa , genetics
A defined mixed culture of Bacillus subtilis and Stenotrophomonas maltophilia was used to accomplish the partial biodegradation of the azo‐dye p ‐aminoazobenzene ( p AAB). Kinetic experiments were conducted, under aerobic conditions, to study the mineralization of p ‐aminoazobenzene by the above‐defined mixed culture, under aerobic conditions. The combination of two previously developed models, (Zissi et al., 1997), which describes p AAB biodegradation by Bacillus subtilis into aniline and p ‐phenylenediamine, and (Zissi and Lyberatos, 1999), which describes aniline biodegradation by Stenotrophomonas maltophilia , is shown to predict well the anticipated mixed culture growth and partial biodegradation of pAAB. In previous work (Zissi et al., 1997) it was observed that p phenylenediamine was unstable during the experiments therefore the fate of p ‐phenylenediamine was not studied. The overall kinetic model of the defined mixed culture was then used to study the behavior of the mixed culture system in a range of operating conditions in the chemostat. The partial degradation of p AAB (regarding one of the two products, aniline) was described by an interaction between the two bacteria with competitive and commensalistic elements. The two bacteria are shown to coexist in a CSTR for some ranges of the operating variables. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 72: 49–54, 2001.