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Succinic acid production with reduced by‐product formation in the fermentation of Anaerobiospirillum succiniciproducens using glycerol as a carbon source
Author(s) -
Lee Pyung Cheon,
Lee Woo Gi,
Lee Sang Yup,
Chang Ho Nam
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/1097-0290(20010105)72:1<41::aid-bit6>3.0.co;2-n
Subject(s) - succinic acid , glycerol , acetic acid , fermentation , chemistry , yield (engineering) , biochemistry , yeast , ethanol , food science , materials science , metallurgy
Succinic acid was produced by fermentation of Anaerobiospirillum succiniciproducens using glycerol as a carbon source. When cells were anaerobically cultured in a medium containing 6.5 g/L glycerol, a high succinic acid yield (133%) was obtained while avoiding the formation of by‐product acetic acid. The gram ratio of succinic acid to acetic acid was 25.8:1, which is 6.5 times higher than that obtained using glucose (ca. 4:1) as a carbon source. Therefore, succinic acid can be produced with much less by‐product formation by using glycerol as a carbon source, which will facilitate its purification. When glucose and glycerol were cofermented with the increasing ratio of glucose to glycerol, the gram ratio of succinic acid to acetic acid and succinic acid yield decreased, suggesting that glucose enhanced acetic acid formation irrespective of the presence of glycerol. Glycerol consumption by A. succiniciproducens required unidentified nutritional components present in yeast extract. By intermittently feeding yeast extract along with glycerol, a high succinic acid yield (160%) could be obtained while still avoiding acetic acid formation. This resulted in the highest ratio of succinic acid to acetic acid (31.7:1). © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 72: 41–48, 2001.