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Synthesis of glycerides containing n‐3 fatty acids and conjugated linoleic acid by solvent‐free acidolysis of fish oil
Author(s) -
Garcia Hugo S.,
Arcos Jose A.,
Ward Derik J.,
Hill Charles G.
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/1097-0290(20001205)70:5<587::aid-bit13>3.0.co;2-h
Subject(s) - lipase , interesterified fat , chemistry , glyceride , candida rugosa , fish oil , fatty acid , conjugated linoleic acid , triacylglycerol lipase , menhaden , linoleic acid , organic chemistry , chromatography , enzyme , fish <actinopterygii> , biology , fishery
Menhaden oil, a rich source of n‐3 fatty acids, was interesterified with conjugated linoleic acid (CLA) in a reaction medium composed solely of substrates and either free or immobilized commercial lipase preparations. Of five lipases tested, an immobilized preparation from Mucor miehei provided the fastest rate of incorporation of CLA into fish oil acylglycerols; however, and as observed with most of the lipases utilized, a significant proportion of the n‐3 fatty acid residues were liberated in the process. A soluble lipase from Candida rugosa converted free CLA to acylglycerol residues while leaving the n‐3 fatty acid residues virtually untouched. Even though the reaction rate was slower for this enzyme than for the other four lipase preparations, the specificity of the free C. rugosa lipase gives it the greatest potential for commercial use in preparing fish oils enriched in CLA residues but still retaining their original n‐3 fatty acid residues. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 70: 587–591, 2000.