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All solid‐state GFP sensor
Author(s) -
Kostov Yordan,
Albano Cornelia Renee,
Rao Govind
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/1097-0290(20001120)70:4<473::aid-bit14>3.0.co;2-2
Subject(s) - cuvette , green fluorescent protein , detection limit , fluorescence , light emitting diode , optoelectronics , light sensitivity , sensitivity (control systems) , diode , materials science , chemistry , optics , physics , chromatography , electronic engineering , biochemistry , engineering , gene
An all‐solid‐state green fluorescent protein (GFP) sensor for GFP measurement was developed. It is immune to interference from ambient light and works with standard flow‐through cuvettes. The sensor is practically insensitive to the scattered excitation light encountered in microbial suspensions. It has a range of 0.0002–1 g/L (7.4 × 10 −9 – 3.7 × 10 −5 M) with limit of detection 0.00019 g/L (7.0 × 10 −9 M). The sensor could be used with a UV or blue light emitting diode (LED) as a light source, depending on required sensitivity, selectivity, and background levels. Its very low cost makes it useful in a variety of applications. This article describes the construction and validation of the sensor both off‐ and on‐line in fermentation processes. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 70: 473–477, 2000.