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High density cultivation of two strains of iron‐oxidizing bacteria through reduction of ferric iron by intermittent electrolysis
Author(s) -
Matsumoto Norio,
Yoshinaga Hisao,
Ohmura Naoya,
Ando Akikazu,
Saiki Hiroshi
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/1097-0290(20001120)70:4<464::aid-bit12>3.0.co;2-a
Subject(s) - ferrous , electrolysis , chemistry , oxidizing agent , ferric , iron bacteria , bacteria , electrolyte , yield (engineering) , strain (injury) , inorganic chemistry , nuclear chemistry , electrode , metallurgy , materials science , biology , organic chemistry , anatomy , genetics
Electrolytic cultivation was applied to Leptospirillum ferrooxidans strains P3A and CF27, which use ferrous iron to respire aerobically. Ferrous iron was supplied to the bacteria by intermittent electrolytic reduction of ferric iron as electron shuttle using an electrode. The yield of L. ferrooxidans and strain CF27 reached 20‐ and 50‐fold, respectively, higher density than were achievable yields without electrolysis. The time required to obtain high density depended not on the growth ratio, but rather on the original growth rate of each strain. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 70: 464–466, 2000.