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Modeling substrate interactions during the biodegradation of mixtures of toluene and phenol by Burkholderia species JS150
Author(s) -
Rogers Julia Bull,
Reardon Kenneth F.
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/1097-0290(20001120)70:4<428::aid-bit8>3.0.co;2-4
Subject(s) - toluene , phenol , biodegradation , kinetics , substrate (aquarium) , chemistry , degradation (telecommunications) , reaction rate constant , organic chemistry , ecology , telecommunications , physics , quantum mechanics , computer science , biology
The biodegradation kinetics of toluene, phenol, and a mixture of toluene and phenol by Burkholderia species JS150 was measured and modeled. Both of these compounds can serve as the sole source of carbon and energy for this microorganism. The single‐substrate biodegradation kinetics was described well using the Monod model, with model constants of μ max,T = 0.39 h −1 and K S,T = 0.011 m M for growth on toluene and μ max,P = 0.309 h −1 and K S,P = 0.0054 m M for growth on phenol. Degradation of the mixture of toluene and phenol followed simultaneous utilization kinetics with toluene being the preferred substrate. Toluene was found to inhibit the rate of utilization of phenol while the presence of phenol had little effect on the rate of degradation of toluene. Of the kinetic models that were tested, one developed for microbial degradation of multiple substrates was able to describe substrate interactions and to model the mixture utilization by strain JS150. Simple competitive, noncompetitive, or uncompetitive substrate kinetics were not sufficient to describe the observed inhibitory interactions. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 70: 428–435, 2000.