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Fungal morphology and fragmentation behavior in a fed‐batch Aspergillus oryzae fermentation at the production scale
Author(s) -
Li Zheng Jian,
Shukla Vivek,
Fordyce Andrew P.,
Pedersen Annemarie Gade,
Wenger Kevin S.,
Marten Mark R.
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/1097-0290(20001105)70:3<300::aid-bit7>3.0.co;2-3
Subject(s) - aspergillus oryzae , fermentation , hypha , mycelium , fragmentation (computing) , industrial fermentation , breakage , biology , bioreactor , chemistry , food science , botany , materials science , composite material , ecology
It is well known that high‐viscosity fermentation broth can lead to mixing and oxygen mass transfer limitations. The seemingly obvious solution for this problem is to increase agitation intensity. In some processes, this has been shown to damage mycelia, affect morphology, and decrease product expression. However, in other processes increased agitation shows no effect on productivity. While a number of studies discuss morphology and fragmentation at the laboratory and pilot scale, there are relatively few publications available for production‐scale fungal fermentations. The goal of this study was to assess morphology and fragmentation behavior in large‐scale, fed‐batch, fungal fermentations used for the production of protein. To accomplish this, a recombinant strain of Aspergillus oryzae was grown in 80 m 3 fermentors at two different gassed, impeller power‐levels (one 50% greater than the other). Impeller power is reported as energy dissipation/circulation function (EDCF) and was found to have average values of 29.3 ± 1.0 and 22.0 ± 0.3 kW m –3 s −1 at high and low power levels, respectively. In all batches, biomass concentration profiles were similar and specific growth rate was < 0.03 h −1 . Morphological data show hyphal fragmentation occurred by both shaving‐off of external clump hyphae and breakage of free hyphae. The fragmentation rate constant ( k frag ), determined using a first‐order model, was 5.90 and 5.80 h −1 for high and low power batches, respectively. At the end of each batch, clumps accounted for only 25% of fungal biomass, most of which existed as small, sparsely branched, free hyphal elements. In all batches, fragmentation was found to dominate fungal growth and branching. We speculate that this behavior was due to slow growth of the culture during this fed‐batch process. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 70: 300–312, 2000.