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Effect of temperature on the inhibition kinetics of phenol biodegradation by Pseudomonas putida Q5
Author(s) -
Onysko Kira A.,
Budman Hector M.,
Robinson Campbell W.
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/1097-0290(20001105)70:3<291::aid-bit6>3.0.co;2-y
Subject(s) - pseudomonas putida , arrhenius equation , biodegradation , kinetics , phenol , chemistry , substrate (aquarium) , yield (engineering) , thermodynamics , bacteria , pseudomonadaceae , pseudomonadales , pseudomonas , organic chemistry , biology , enzyme , physics , ecology , activation energy , genetics , quantum mechanics
Abstract The temperature‐dependent performance of mixed‐culture wastewater treatment processes may be strongly influenced by their content of psychrotrophic bacteria. In this work, the effect of temperature on cell growth and phenol biodegradation kinetics of the psychrotrophic bacterium Pseudomonas putida Q5 were determined using both batch and continuous cultures in the range of 10–25°C. The Haldane equation was found to be the most suitable substrate‐inhibition model for the specific growth rate. The Haldane parameters μ max and K I were best modeled by a square‐root dependency on temperature. However, the Arrhenius model provided a better prediction of the temperature dependence of K S . The variation of the yield constant with temperature also was studied experimentally. Comparisons with results of previous workers are presented. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 70: 291–299, 2000.