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The growth of single roots of Artemisia annua in nutrient mist reactors
Author(s) -
Wyslouzil Barbara E.,
Waterbury Raymond G.,
Weathers Pamela J.
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/1097-0290(20001020)70:2<143::aid-bit3>3.0.co;2-b
Subject(s) - nutrient , mist , artemisia annua , environmental science , chemistry , biology , ecology , geography , artemisinin , meteorology , malaria , immunology , plasmodium falciparum
To better characterize the development and growth of hairy roots in a mist‐fed root bed, a single root aerosol reactor was developed. Growth kinetics studies were conducted on hairy roots of Artemisia annua as a function of the mist cycle, carrier gas, and nutrient compositions. Sustained rapid growth was only observed when conditioned medium was fed to the roots. The presence of 1% CO 2 in the carrier gas did not enhance the growth kinetics but it did prevent necrosis of the tissue at the highest mist cycle. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 70: 143–150, 2000.