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Feasible boundaries of aqueous two‐phase systems with NH 3 and CO 2 as recyclable volatile salts
Author(s) -
van Berlo Mos,
Ottens Marcel,
Luyben Karel Ch. A. M.,
van der Wielen Luuk A. M.
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/1097-0290(20001005)70:1<65::aid-bit8>3.0.co;2-a
Subject(s) - limiting , aqueous solution , salt (chemistry) , chemistry , phase (matter) , peg ratio , ammonia , carbon dioxide , extraction (chemistry) , polymer , work (physics) , carbon fibers , chemical engineering , process engineering , organic chemistry , computer science , thermodynamics , mechanical engineering , physics , finance , engineering , algorithm , composite number , economics
Aqueous two‐phase systems (ATPSs) have great potential for use in the downstream processing of fermentation products. A major drawback of these systems, limiting application in industrial practice up till now, is the consumption of large amounts of auxiliary materials such as polymers and salts. Making use of alternative auxiliaries can diminish this relatively large discharge. A possible approach is to make use of volatile salts induced by combinations of ammonia and carbon dioxide that can be recycled to the extraction system. As part of an ongoing research effort on ATPSs with volatile salts, this work aims at getting more information on the system boundaries or operating conditions of these systems in terms of phase behavior. The results show that the NH 3 /CO 2 ratio is an important parameter that has a large influence on the system boundaries. Both for systems with PEG 2000 and PEG 4000, this ratio has to be larger than about 1.75 to make a liquid–liquid phase separation possible. The most optimal ratio seems to be 2.0 for reasons of solution composition and absence of solid salt. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 70: 65–71, 2000.