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Biotechnology for the production of nutraceuticals enriched in conjugated linoleic acid: II. Multiresponse kinetics of the hydrolysis of corn oil by a Pseudomonas sp. lipase immobilized in a hollow‐fiber reactor
Author(s) -
Sehanputri Prima S.,
Hill Charles G.
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/1097-0290(20000820)69:4<450::aid-bit11>3.0.co;2-v
Subject(s) - chemistry , hydrolysis , conjugated linoleic acid , lipase , chromatography , bioreactor , fatty acid , corn oil , linoleic acid , triacylglycerol lipase , organic chemistry , enzyme , food science
The hydrolysis of corn oil in the presence of a lipase from Pseudomonas sp. immobilized within the walls of a hollow‐fiber reactor was studied at 30°C. To assess the selectivity of this immobilized enzyme, the effluent concentrations of five different free fatty acids were measured using high‐performance liquid chromatography (HPLC). Several rate expressions associated with a generic ping‐pong bi‐bi mechanism were used to fit the experimental data for this lipase‐catalyzed reaction. A multiresponse nonlinear regression method was employed to determine the kinetic parameters associated with these rate expressions. Quasi‐optimum operating conditions corresponded to 30°C and a buffer pH value of 7.0. Under these conditions, the concentration of free linoleic acid (C18:2) (the fatty acid of primary interest) in the effluent oil stream for a fluid residence time of 6 h was approximately 0.5 M . © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 69: 450–456, 2000.