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Microbial dynamics in a continuous stirred tank bioreactor exposed to an alternating sequence of organic compounds
Author(s) -
Jorge Ruben Miguel Ferreira,
Livingston Andrew Guy
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/1097-0290(20000820)69:4<409::aid-bit7>3.0.co;2-d
Subject(s) - bioreactor , biodegradation , degradation (telecommunications) , continuous flow , chemistry , strain (injury) , microbial biodegradation , environmental chemistry , microorganism , chromatography , bacteria , biology , organic chemistry , biochemical engineering , telecommunications , genetics , computer science , engineering , anatomy
Microbial dynamics during aerobic biodegradation of an alternating mixture of organic compounds was investigated experimentally in a continuous stirred tank bioreactor (CSTB). A mathematical model describing this system was developed and tested using the experimental results. A model microbial culture consisting of Pseudomonas sp. JS150, a monochlorobenzene (MCB) degrader, and Xanthobacter autotrophicus GJ10, a 1,2‐dichloroethane (DCE) degrader, each with exclusive degradation capabilities, was used. The CSTB was inoculated with both microbial strains and exposed to an alternating sequence of the two compounds at noninhibitory concentrations. Concentrations of each microbial strain, of each organic compound, and of degradation product evolved, as well as specific microbial activities via oxygen uptake tests, were monitored. Reduction of the residual DCE discharged from the bioreactor after an MCB to DCE transition was successfully achieved by continuously feeding a low flow of a concentrated solution of both compounds. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 69: 409–417, 2000.