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A two‐reservoir, hollow‐fiber bioreactor for the study of mixed‐population dynamics: Design aspects and validation of the approach
Author(s) -
Manjarrez Edgar Salgado,
Albasi Claire,
Riba JeanPierre
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/1097-0290(20000820)69:4<401::aid-bit6>3.0.co;2-3
Subject(s) - bioreactor , mixing (physics) , dynamics (music) , population , membrane bioreactor , biochemical engineering , fiber , biological system , computer science , chemistry , engineering , biology , physics , demography , organic chemistry , quantum mechanics , sociology , acoustics
A two‐reservoir, membrane bioreactor for carrying out studies of mixed‐population dynamics in batch fermentations is presented. Mixing requirements and design aspects for the validity of the approach are given and discussed. Equations describing mixing times between the reservoirs are presented and compared to the experimental results. The validity of the approach is demonstrated by the study of an amensalistic‐type interaction, the protein‐mediated killer phenomenon between two Saccharomyces cerevisiae strains. The validation consisted in the comparison between the results obtained in actual mixed culture and the results obtained by keeping the strains separated. A good agreement was found which demonstrates the viability of the designed bioreactor. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 69: 401–408, 2000.