Premium
Detection of toxic chemicals with high sensitivity by measuring the quantity of induced P450 mRNAs based on surface plasmon resonance
Author(s) -
Oyama Masaaki,
Ikeda Takeshi,
Lim Taekyu,
Ikebukuro Kazunori,
Masuda Yuzo,
Karube Isao
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/1097-0290(2000)71:3<217::aid-bit1011>3.0.co;2-w
Subject(s) - surface plasmon resonance , peptide nucleic acid , nucleic acid , nasba , biosensor , chemistry , dna , chromatography , microbiology and biotechnology , biochemistry , biology , nanotechnology , materials science , nucleic acid sequence , nanoparticle
In this study we describe a novel sensor system to detect toxic chemicals based on measurement of the quantity of Saccharomyces cerevisiae P450 mRNAs induced by them. Detection was conducted using a flow‐injection‐type sensor system based on surface plasmon resonance (SPR). The DNA and peptide nucleic acid (PNA) probes containing a complementary sequence to a part of P450 mRNA were immobilized on the sensor chip and the P450 mRNAs hybridized to the probes were quantified. We succeeded in detecting 10 ng/L (10 ppt) of atrazine using both DNA and PNA probes. Using this sensor system, we were able to detect bisphenol A in addition to atrazine. Furthermore, we achieved higher sensitivity by amplifying the target P450 mRNA based on nucleic acid sequence‐based amplification (NASBA). This method allows for sensitive, rapid, and easy detection of some toxic chemicals. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 71: 217–222, 2000/2001.