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Use of lipophilic anions for estimation of biomass and cell viability
Author(s) -
Daugelavičius Rimantas,
Bakienė Elena,
Beržinskienė Janina,
Bamford Dennis H.
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/1097-0290(2000)71:3<208::aid-bit1010>3.0.co;2-0
Subject(s) - enumeration , population , biomass (ecology) , microorganism , cell , chemistry , biology , bacteria , biochemistry , microbiology and biotechnology , genetics , ecology , demography , mathematics , combinatorics , sociology
A method is described to estimate the microbial biomass of a sample, to enumerate the cells, and to distinguish the portion of metabolically active cells in the population by measuring the binding of phenyldicarbaundecaborane (PCB − ) to the cells. This method can also be used for the analysis of a complex population of microorganisms if the cells composing the sample are sensitive to different biocidal agents. In addition, the analysis of PCB − binding is useful for the enumeration of the phage‐infected cells and phage particles. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 71: 208–216, 2000/2001.