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High resolution MAS‐NMR in combinatorial chemistry
Author(s) -
Shapiro Michael J.,
Gounarides John S.
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/1097-0290(2000)71:2<130::aid-bit1004>3.0.co;2-r
Subject(s) - magic angle spinning , nuclear magnetic resonance spectroscopy , chemistry , spinning , magnetic dipole–dipole interaction , spin diffusion , solid state nuclear magnetic resonance , polymer , resolution (logic) , nmr spectra database , magic angle , analytical chemistry (journal) , nuclear magnetic resonance , spectral line , diffusion , dipole , organic chemistry , physics , polymer chemistry , computer science , astronomy , artificial intelligence , thermodynamics
High‐resolution magic angle spinning (hr‐MAS) NMR is a powerful tool for characterizing organic reactions on solid support. Because magic angle spinning reduces the line‐broadening due to dipolar coupling and variations in bulk magnetic susceptibility, line widths approaching those obtained in solution‐phase NMR can be obtained. The magic angle spinning method is amenable for use in conjunction with a variety of NMR‐pulse sequences, making it possible to perform full‐structure determinations and conformational analysis on compounds attached to a polymer support. Diffusion‐weighted MAS‐NMR methods such as SPEEDY (Spin‐Echo‐Enhanced Diffusion‐Filtered Spectroscopy) can be used to remove unwanted signals from the solvent, residual reactants, and the polymer support from the MAS‐NMR spectrum, leaving only those signals arising from the resin‐bound product. This review will present the applications of high‐resolution magic angle spinning NMR for use in combinatorial chemistry research. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng (Comb Chem) 71:130–148, 2000/2001.